poly di- dc 20148e (Thermo Fisher)
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poly di- dc 20148e
Poly Di Dc 20148e, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly(di-dc)+20148e/poly+di+dc+/pm40542553-72-39-45
Average 90 stars, based on 1 article reviews
Poly Di Dc 20148e, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly(di-dc)+20148e/poly+di+dc+/pm40542553-72-39-45
Average 90 stars, based on 1 article reviews
poly di- dc 20148e - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Dominant Malignant Clones Leverage Lineage Restricted Epigenomic Programs to Drive Ependymoma Development Article Snippet: A DNA library (Integrated DNA Technologies) containing randomized central regions of 20 bp flanked by constant sequences complimentary to primers was converted to dsDNA and brought to 74ng/μL before being combined 1% w/v BSA, 500ng/μL Article Title: Functional Characterization of the Gibberellin (GA) Receptor ScGID1 in Sugarcane Article Snippet: Typical binding reactions contained 150 ng Article Title: Functional Characterization of the Gibberellin (GA) Receptor ScGID1 in Sugarcane. Article Snippet: Typical binding reactions contained 150 ng Article Title: cAMP-Response Element (CRE)-Mediated Transcription by CRE-Binding Protein (CREB) Is Essential for Human Tryptophan Hydroxylase 2 Gene Expression. Article Snippet: For TPH2- CRE EMSA, nuclear proteins (6.2 μg) were incubated at 4°C for 30 min in 20- μL reaction mix containing 5% glycerol, 50 mM NaCl, 5 mM MgCl2, 10 mM Tris– HCl buffer (pH 7.5), and 50 μg/mL Article Title: Alternate isoforms of IRF7 Differentially Regulate Interferon Expression to Tune Response to Viral Infection Article Snippet: The EMSA binding buffer consisted of 20 mM Tris-HCl (pH 7.5), 1 mM EDTA, 20 mM KCl, 1 mM MgCl2, 10% glycerol, 5 mM DTT, 0.5% NP-40, 10 μg of BSA, and 62.5 μg/mL Poly( Article Title: A humanized NOVA1 splicing factor alters mouse vocal communications Article Snippet: Purified NOVA1 protein (0.5–2 pmol/reaction) and labeled RNA probe (0.08 pmol/reaction) were mixed under the following buffer conditions: 10 mM HEPES, 3 mM MgCl2, 150 mM NaCl, 5% Glycerol, 0.1 mM DTT, 0.1U/μl Article Title: DNA-guided transcription factor cooperativity shapes face and limb mesenchyme. Article Snippet: Binding reactions were set up by diluting 0.5 ul Protease Inhibitor:Article Title: Efficient proteome‐wide identification of transcription factors targeting <i>Glu‐1</i>: A case study for functional validation of <scp>TaB3‐2A1</scp> in wheat Article Snippet: .. DNA pull-down assays were performed in four steps (IIV) as follows: I, preparing beads: (1) pipet 250 lL Dynabeads M-280 Streptavidin (Cat#11205D, Invitrogen, Shanghai, China) into a fresh microfuge tube and vigorously shake Dynabeads to resuspend in the company-supplied preservative; (2) secure the microfuge tube on a PolyATract 1000 magnet (Cat#CS15000, Promega) to pull Dynabeads down; (3) wash the beads with 500 lL of 2 9 B/W buffer [10 mM Tris–HCl (pH 7.5), 1 mM EDTA, 2 M NaCl] for three times; II, assembling probe-beans: (1) resuspend the beads in 190 lL of 2 9 B/W buffer and add 200 lL biotinylated probe (200–400 ng/ll); (2) incubate the mixture while rolling at room temperature for 20 min; (3) pull beads down by applying to the PolyATract 1000 magnet; (4) repeat the steps (1)–(3) to ensure that the beads are saturated with DNA; (5) wash the probe-beads with 500 lL of BS/THES buffer [22 mM Tris–HCl (pH 7.5), 4.4 mM EDTA, 8.9% sucrose (m/v), 62 mM NaCl, 0.3% protease inhibitor, 0.04% phosphatase inhibitor, 10 mM HEPES, 5 mM CaCl2, 50 mM KCl, 12% glycerol] to ensure that the DNA probe is in the reaction conditions suitable for DNA-protein interactions; III, capturing target proteins binding to the probe: (1) apply 200 lL of BS/ THES buffer to probe-bead complex along with 600–750 lL of cleared lysate (supernatant); (2) add 25–100 lg of Binding Assay:Article Title: Efficient proteome‐wide identification of transcription factors targeting <i>Glu‐1</i>: A case study for functional validation of <scp>TaB3‐2A1</scp> in wheat Article Snippet: .. DNA pull-down assays were performed in four steps (IIV) as follows: I, preparing beads: (1) pipet 250 lL Dynabeads M-280 Streptavidin (Cat#11205D, Invitrogen, Shanghai, China) into a fresh microfuge tube and vigorously shake Dynabeads to resuspend in the company-supplied preservative; (2) secure the microfuge tube on a PolyATract 1000 magnet (Cat#CS15000, Promega) to pull Dynabeads down; (3) wash the beads with 500 lL of 2 9 B/W buffer [10 mM Tris–HCl (pH 7.5), 1 mM EDTA, 2 M NaCl] for three times; II, assembling probe-beans: (1) resuspend the beads in 190 lL of 2 9 B/W buffer and add 200 lL biotinylated probe (200–400 ng/ll); (2) incubate the mixture while rolling at room temperature for 20 min; (3) pull beads down by applying to the PolyATract 1000 magnet; (4) repeat the steps (1)–(3) to ensure that the beads are saturated with DNA; (5) wash the probe-beads with 500 lL of BS/THES buffer [22 mM Tris–HCl (pH 7.5), 4.4 mM EDTA, 8.9% sucrose (m/v), 62 mM NaCl, 0.3% protease inhibitor, 0.04% phosphatase inhibitor, 10 mM HEPES, 5 mM CaCl2, 50 mM KCl, 12% glycerol] to ensure that the DNA probe is in the reaction conditions suitable for DNA-protein interactions; III, capturing target proteins binding to the probe: (1) apply 200 lL of BS/ THES buffer to probe-bead complex along with 600–750 lL of cleared lysate (supernatant); (2) add 25–100 lg of |